View source for Equation 4

[[Category:Sednterp]]The reduced apparent molecular weight, σ, is directly available from sedimentation equilibrium: Equating [[equation 1]] and [[equation 2]], one obtains the fundamental equation describing sedimentation equilibrium of a single, thermodynamically ideal protein:
equation 4.

[[File:Equation4-a.GIF]]

where σ is the reduced apparent molecular weight (Ref. [[14]] [[25]]), c(r) is the weight concentration at radial position r, and the other terms are as described above or in the [[table of symbols]]. While σ may be determined from the slope of a graph of ln c as a function of (r^2)/2, sedimentation equilibrium data is analyzed best by nonlinear least squares procedures, which can accommodate more complicated cases involving association and nonideality (Ref. [[2]] [[10]] [[14]] [[26]]).