Table of Contents

I. Introduction.

A. Simple Step Through.

B. Overview.

1. General Information on Sednterp.

2. General Glossary.

3. Table of symbols Used.

C. Features of Sednterp Program.

1. List of Parameters for Input.

2. List of Interpolations Available.

3. List of Results.

4. List of Graphs.

5. List of Standard File Endings.

D. Files Necessary to Run Sednterp.

E. Development and Distribution of Sednterp.

F. Who to Contact for Information and Feedback.

G. Acknowledgments.

II. Theory of Computer Aided Interpretation of Sedimentation Data.

A. Primary Reference.

B. Introduction.

C. Background.

1. Overview of Information Needed.

2. Sedimentation Coefficient.

3. Reduced Apparent Molecular Weight:

4. Buffer and Solute Solution Parameters.

5. Limitations.

D. Calculations Implemented in Sednterp.

1. Molecular Weight.

2. Buoyant Molecular Weight.

3. Calculating v-bar estimates.

4. Calculating Denatured v-bar Estimates.

5. Calculating the Density of the Buffer.

a. Calculating Density Increments.

1) Buffer components.

2) Isotopes of water.

b. Calculating the Density Temperature Correction.

6. Calculating Viscosity.

7. Converting s to s-20-w.

8. Calculating the Equivalent Radius.

9. Estimation of Protein Hydration.

a. Limitations on Estimation of Hydration and Asymmetry.

b. Calculating Maximum Hydration.

c. Estimating Hydration.

d. Calculating Hydration from Asymmetry Estimates.

10. Estimation of Protein Asymmetry.

a. Calculating Ellipsoid Asymmetry.

b. Calculating Cylindrical Asymmetry.

11. Calculating the Stokes Radius

E. References

III. Sednterp Program Control and Screens.

A. Overview of Sednterp Screens.

B. Programming Strategy.

1. Sednterp Uses Default Values.

2. The User Controls Program Information and Flow.

3. A Sparse Main Form Leads to Other Program Elements.

4. Description of the Access Databases

C. The Main Form.

1. The File Menu.

2. The Results Menu.

3. The Estimating Databases Menu.

4. The Experiment Section.

5. The Sample Section.

6. The Buffer Section.

D. Other Forms.

1. The Saving Data in Database Form.

2. The Results Form.

3. The Sample Composition Form.

4. Buffer Components Form.

E. Graphs Available.