[[Category:Sednterp]] [[Category:Table of Contents]]
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[[Category:Sednterp]]'''I. Introduction.'''
1. [[General Information on Sednterp]].
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[[Category:Sednterp]][[Category:Introduction]]
In this section of the help file, basic instructions for the use of Sednterp are given. It is assumed that the user reading these instructions is somew
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[[Category:Sednterp]][[Category:Introduction]]
The purpose of Sednterp is to automatically preform calculations on data connected to ultracentrifu
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[[Category:Sednterp]][[Category:Introduction]]
Sednterp can compute the molecular weight from:
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[[Category:Sednterp]]
...mpute the weight of a protein from its composition. To use this method in Sednterp, press the From Composition button (alt-c shortcut keystroke). The Sample
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[[Category:Sednterp]]
...dard tables of single component buffer data have been fit by polynomials. Sednterp computes density increments for each component at the user entered concentr
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[[Category:Sednterp]]The first step Sednterp uses to calculate the density of a buffer is to calculate the density of th
...rom the most parsimonious polynomial providing an adequate fit are used by Sednterp. Adequacy of the fits were judged by the following criteria: 1) the r.m.s.
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[[Category:Sednterp]]The sample composition form allows the user to enter the composition of a
...atibility with pre-release versions of Sednterp. With current versions of Sednterp it is not necessary to save composition data in such files, since the compo
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[[Category:Sednterp]][[Category:Sednterp Program Control and Screens]]
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[[Category:Sednterp]]
...tal information, but instead contains essential extrapolation data for the Sednterp program. Users can create their own experimental information databases in
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[[Category:Sednterp]]In this form, example, sample, buffer, or results information is saved as
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[[Category:Sednterp]]
...tal information, but instead contains essential extrapolation data for the Sednterp program. Users can create their own experimental information databases in
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[[Category:Sednterp]]
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[[Category:Sednterp]]The partial specific volume is a measure of the change in the volume (in
...and the carboxy-terminal hydroxyl (and that is the implementation used in Sednterp).
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[[Category:Sednterp]][[Category:Introduction]]
...proteins from the composition of the protein and its ligands. Optionally, Sednterp will also compute the molecular weight from this also.
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[[Category:Sednterp]][[Category:Introduction]]
...density and viscosity data has not yet been fit to the polynomial function Sednterp uses to interpolate these values. With experimental measurements, new comp
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[[Category:Sednterp]][[Category:Introduction]]
...an average estimating formula worked out by Teller (Ref. [[28]] [[33]]). Sednterp automatically calculates the equivalent radius when [[Mr is computed]]. It
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[[Category:Sednterp]]Sednterp can compute the molecular weight from:
Sednterp will let you [[enter a molecular weight]] yourself to use in latter computa
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[[Category:Sednterp]]
Given a certain temperature, rotor speed, and σ, Sednterp will use default approximate values of vbar and ρ automatically to compute
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[[Category:Sednterp]]The reduced apparent molecular weight, σ, is directly available from sedi
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[[Category:Sednterp]]
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[[Category:Sednterp]]
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[[Category:Sednterp]][[Category:Introduction]]
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[[Category:Sednterp]]
...proteins from the composition of the protein and its ligands. Optionally, Sednterp will also compute the molecular weight from this also.
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[[Category:Sednterp]]
...density and viscosity data has not yet been fit to the polynomial function Sednterp uses to interpolate these values. With experimental measurements, new comp
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[[Category:Sednterp]]
For those cases when the molecular weight of a sample is known, Sednterp allows the user to enter a molecular weight directly. Click on the Exact M
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[[Category:Sednterp]][[Category:Theory of Computer Aided Interpretation of Sedimentation Data]]
...if some information is available about either hydration or asymmetry then Sednterp will use this to calculate better estimates of the other value. For instanc
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[[Category:Sednterp]]
...an average estimating formula worked out by Teller (Ref. [[28]] [[33]]). Sednterp automatically calculates the equivalent radius when [[Mr is computed]]. It
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[[Category:Sednterp]]
...if some information is available about either hydration or asymmetry then Sednterp will use this to calculate better estimates of the other value. For instanc
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[[Category:Sednterp]]To interpolate a density or viscosity press the compute button on either t
...density and viscosity data has not yet been fit to the polynomial function Sednterp uses to interpolate these values. With experimental measurements, new comp
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[[Category:Sednterp]]
...ll as the protein's partial specific volume. Such data may be entered into Sednterp directly (and temperature corrections computed) or the program may use vari
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[[Category:Sednterp]][[Category:Introduction]]
In the program Sednterp, interpretation refers to the computation of molecular structural propertie
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[[Category:Sednterp]]
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[[Category:Sednterp]][[Category:Introduction]]
Results are molecular parameters computed by Sednterp from the equations described in the theory section. To understand the resu
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[[Category:Sednterp]]
...uses a power series approximation of standard tabular data. For example, Sednterp will interpolate the density of a solution from the concentration of solute
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[[Category:Sednterp]][[Category:Introduction]]
Sednterp will interpolate values for the following properties.
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[[Category:Sednterp]]
...and the carboxy-terminal hydroxyl (and that is the implementation used in Sednterp).
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[[Category:Sednterp]][[Category:Introduction]]
...atibility with pre-release versions of Sednterp. With current versions of Sednterp it is not necessary to save composition data in such files, since the compo
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[[Category:Sednterp]]
...dard tables of single component buffer data have been fit by polynomials. Sednterp computes density increments for each component at the user entered concentr
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[[Category:Sednterp]]
The first step Sednterp uses to calculate the density of a buffer is to calculate the density of th
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[[Category:Sednterp]]
Sednterp can interpolate the viscosity of a buffer from viscosity table data in the
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[[Category:Sednterp]][[Category:Theory of Computer Aided Interpretation of Sedimentation Data]]
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[[Category:Sednterp]][[Category:Introduction]]
...ysical parameters of common buffers used in sedimentation experiments. The Sednterp program was developed at AMGEN by John Philo with the help of David Hayes a
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[[Category:Sednterp]][[Category:Introduction]]
The Sednterp program helps to interpret σ's found by other programs (such as NONLIN). T
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[[Category:Sednterp]][[Category:Introduction]]
The Sednterp program requires the weight of the solute and s for various shape estimates
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[[Category:Sednterp]][[Category:Introduction]]
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[[Category:Sednterp]][[Category:Introduction]]
...p uses a power series approximation of standard tabular data. For example, Sednterp will interpolate the density of a solution from the concentration of solute
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[[Category:Sednterp]][[Category:Introduction]]
...g users to compile reports and make use of the data in programs other than Sednterp.
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[[Category:Sednterp]][[Category:Introduction]]
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